Introducing a New Approach to Genetic Screening: HRM Analysis on the Corbett Rotor-Gene 6000

The identification of genetic variations in microorganisms has become increasingly important in clinical laboratories, particularly when it comes to detecting closely related species. Traditional methods such as DNA sequencing and multiplex PCR can be time-consuming and prone to errors. However, a new approach has emerged that offers a faster and more reliable alternative: high-resolution melting (HRM) analysis on the Corbett Rotor-Gene 6000.

Unlike traditional methods, HRM does not require multiplex methods or DNA sequencing, making it a simpler and more rapid way to detect genetic variations. The probe-free HRM assay also reduces the risk of PCR carryover contamination. In fact, HRM can detect parasites within just 30 minutes after real-time PCR detection, making it an ideal tool for clinical laboratories.

The Corbett Rotor-Gene 6000 instrument is capable of performing HRM analysis using a range of primers, including those specific to the HhaI repetitive region (HR), small heat shock protein (SHP), small subunit ribosomal DNA (18S rDNA), internal transcribed spacer region (ITS), and trans-spliced leading Exon I gene (SLX1). Using these primers, researchers can identify two closely related species of Brugia, B. malayi and B. pahangi.

In a recent study, HRM analysis was used to test five Brugia specimens using the Corbett Rotor-Gene 6000 instrument. The results showed that HRM analysis on the ITS and SLX genes clearly generated two profiles of B. pahangi, allowing for accurate identification of these closely related species.

The software used to run the HRM analysis is Rotor-Gene Q v2.3 or higher, which is compatible with Windows XP and Windows 7 operating systems (32-bit and 64-bit). This makes it easy for researchers to integrate HRM into their workflow, regardless of their computer setup.

In summary, HRM analysis on the Corbett Rotor-Gene 6000 offers a new approach to genetic screening that is faster, simpler, and more reliable than traditional methods. Its ability to detect closely related species quickly and accurately makes it an ideal tool for clinical laboratories.

Ncbi Corbett Rotor Gene 6000